Dna binding studies of metal complexes pdf download

Spectroscopic, microbial and dna binding affinities of. These studies are necessary for the further comprehension of binding of transition metal complexes to dna. The complexes are characterized by ftir, uvvisible and mass spectral studies and assessed for their dna binding study using. Synthesis, characterization, and dna binding studies of. The dna binding properties of these complexes were searched for the interaction with ct dna by electronic absorption titration and emission titration. Biomolecular interaction, anticancer and antiangiogenic. Thus, the current study is focused on synthesis of zinc ii. Dna binding studies were carried out by viscosity method. Competitive dnabinding studies between metal complexes and. In modern medicinal chemistry, transition metal complexes for their binding properties has become suitable candidates as anticancer and antitumor drugs, the most important examples are cisplatin, carboplatin, and oxaliplatin. In vitro study on dna binding of rutheniumii complexes with. May 29, 2011 the structural features of schiff base and metal complexes were determined from their elemental analyses, thermogravimetric studies, magneticsusceptibility, molar conductivity, esimass, ir, uvvis, 1 h nmr and esr spectral studies. The method is based on the decrease of fluorescence derived from the displacement of gelred from dna by metal complexes.

Pdf synthesis, characterization, antimicrobial, dna. Synthesis, characterization, dna binding and cleavage. Three complexes of an ons donor ligand of the schiff base c21h19no2s hl, cuc21h18no2s2 1 coc21h18no2s2 2 and pdc21h18no2scl 3, w. The dna binding and cleavage study of the copper complex containing the ligand is reported. The binding poses of the metal complexes 1 c and 2 d into the bsa obtained.

An investigation of new metal complexes by using various metals and ligands is necessary to understand role and importance of the effect factors on the dna binding. Dec 14, 2011 octahedral transition metal complexes containing three bipyridine or phenanthroline ligands have been shown to be groove binders or possible partial intercalators 8, 9. The ligand and their metal complexes were screened for their antimicrobial activity against bacteria. The observed value for pln closely matches with the previous report and that of npn1 showed much higher value than pln and its copper complexes due to its better solubility in aqueous medium than its counterparts. Three dimensional representations of various metal complex geometries. This work was completed in collaboration with prof. From the spectral studies an octahedral geometry was proposed for the copper complex. Enantiomeric recognition of chiral l and dpenicillamine. Biological studies of copperii schiff base complexes. Complexes vs ct dna binding studies were investigated by uvvis spectroscopy. Truly, metal complexes bound to dna provide an interesting system to study structure, function and dynamics in a single package. Metal complexes coii,cuii and znii ions of schiff base having played a central role in the development of coordination chemistry. Pdf dna binding, amelioration of oxidative stress, and.

Protein binding, dna bindingcleavage and in vitro cytotoxicity studies of 23dimethylaminopropylaminonaphthalene1,4dione l and its four coordinated. Download hires imagedownload to mspowerpointcite this. A series of mono and bimetallic metal complexes with cuii, ptii and znii with substituted polypyridyl ligands have been prepared and their binding affinities towards quadruplex cmyc and human telomeric and duplex dna ds26 and calf thymus determined using fluorescent indicator displacement fid assa metal interactions with nucleic acids. Structural data of dna binding and molecular docking. Design, syntheses and functional studies of dna binding. Synthesis, characterization, antimicrobial, dna binding and cleavage studies of schiff base metal complexes. Dna cleavage ability of the complexes was examined on pbr322 dna by using gel electrophoresis method. Structurefunction relationship in dnabinding proteins.

Synthesis, characterization, antimicrobial, dna binding. These aromatic side arms introduce new modes of dna binding, involving mutual interactions of functional groups held in close proximity. Metal complex interactions with dna dalton transactions. The more negative relative binding energy of lform of complex suggests greater propensity for dna than the denantiomer. Based on these reports, the synthesized copperii schiff base complexes were screened to know whether these complexes have any cytotoxic and dna binding activities. The relative binding efficacy of the complexes to dna was much higher than the binding of ligands, especially the complex of cu l1 l2 cl 2 had the highest binding ability to dna. Complexes, both new and old, are currently being utilised in conjunction with spectroscopic and biological techniques to probe the interactions of dna and its many structural forms. Dnabinding and photocleavage studies of cobalt iii. Dna and bsabinding studies and anticancer activity against.

The complexes were screened for their antimicrobial activity. Dna binding, amelioration of oxidative stress, and molecular docking study of znii metal complex of a new schiff base ligand. Dna cleavage, binding and intercalation studies of drug. The binding affinities of dna metal complexes are a key issue for understanding the mechanism of effective metal based chemotherapeutic drugs. Cleavage reactions were run between the metal complexes and dna. Dna and bsabinding studies and anticancer activity. Design, syntheses and functional studies of dna binding metal. Synthesis, characterization, biological activity and dna binding. In the recent years, the interaction between transition metal complexes with dna and proteins have been intensively studied. Synthesis, characterization, electrochemical studies, dna binding, and cleavage activity of mixed copperiioxime dmso complexes. Dna binding properties of these metal complexes were investigated using absorption spectroscopy. The binding affinities of these complexes with calf thymus dna are also reported. Their crystal structures were deterimined by singlecrystal xray diffraction method. In addition, the dnabinding properties of the niii complex have been.

Binding studies of 4, 5a, and 5b against calf thymus dna were carried out, revealing that 4, 5a, and 5b have. For example, square planar complexes penetrate deeper into the dna. Synthesis, characterization, biological activity and dna. Design, syntheses and functional studies of dna binding metal complexes final seminar april 10, 1998 antitumor activity of many anticancer drugs is based on their interaction with dna five basic interaction types are observed. Dna binding and cleavage studies of two palladiumii. Quantum chemical studies of metaldna interactions orca. Synthesis, characterisation and dna binding studies of metal. From all the spectral data a distorted octahedral geometry has proposed for both the complexes.

Furthermore, in the case of vanadium, many studies on its interaction with dna have been performed. Reactivity and structural studies of metal complexes bound to. Synthesis, dna binding, and antimicrobial studies of novel metal. Introduction the schiff bases are an important class of ligands in coordination chemistry. Using fret melting assays, the binding affinity and selectivity of the five metal complexes against six different g4 dna structures as well as a duplex dna. Synthesis, crystal structure, and dnabinding studies of a nickelii. Transition metal complexes play an important role in nucleic acids chemistry for their diverse. Synthesis and dna binding studies of niii, coii, cuii and znii metal complexes of n1,n5bispyridine2methylenethiocarbohydrazone schiffbase ligand. We have also assessed the dna binding efficacy and mode of binding of both the complexes. Synthesis, characterization, biological activity and. Studies on the interaction of the metal complex of. Synthesis, characterization, and dna binding properties of a.

Transition metal ions, mixed ligands, copper complexes, dna binding. Studies of dna binding of lanthanide platinum complexes. They also can be served as complementary studies for the corresponding complexes of ruthenium. Hence, we report synthesis and characterization of novel schiff base ligand l and its complexes with cuii. Synthesis, characterization, antimicrobial, dna binding and cleavage studies of mixed ligand cuii, coii complexes. The characterization studies of all the metal complexes have been done with appropriate methods. Synthesis, characterization, antifungal, antibacterial and. Concerning the noncovalent interactions between transition metal complexes and dna, they can occur by intercalation, groove binding, or external electrostatic binding. Pdf synthesis and dna binding studies of ni ii, co ii. Looking to the above important of dna binding study of ligands and metal complexes 12, the present paper describes mixed ligand synthesis and dna binding interaction assay of l1 and l2. Jcprc5 1159 binding studies of some novel macroacyclic transition metal complexes towards ct dna via multispectroscopic techniques c. Several studies were carried out to elucidate the detailed binding mode between this family of complexes and quadruplex dna. The interaction between the metal complex of hydrazide of podophyllic acid and calf thymus ct dna was studied by using absorption spectra, fluorescence spectra and dna heat denaturation. New homodiand heterotrinuclear metal complexes of schiff base.

The results showed that the interaction between ndiii complex and ct dna by surfaceelectrostatic binding, while all the other complexes can bind to calfthymus dna mainly by intercalation mode. All the complexes 14 show a bathochromic and hyperchromic shift with. Synthesis, characterization and dna binding study of mixed. A novel technique for distinguishing the binding locations of metal complexes in dna. Transition metal complexes derived from n, s bidentate ligands. The results suggest that both complexes bind to dna through intercalation and enantioselectively interact with ctdna.

Also, bioactive metal complexes are useful biochemical tools for the detection of dna both in vitro and inside the cell, as dna probes 14, 15. Synthesis, spectral, dna binding and cleavage properties of. Binding studies of metalsalphen and metalbipyridine. Mass spectrometric studies of alkali metal ion binding on thrombin binding aptamer dna eun sun hong, ahyejoo yoon, byungjoo kim, byonghyeon yim, hunyoung so,b and seung koo shina a bionanotechnology center, department of chemistry, pohang university of science and technology, pohang, korea b division of metrology for quality life, korea research institute of standards and. Thus, the current study is focused on synthesis of zinc ii complexes using amino acid schiff bases as ligands. Lawson in the department of chemistry, state university of new york college at fredonia. Experimental results indicated that complexes and cipro. Pdf the interaction of ligands and their transition metal complexes with plasmid puc19 dna and calf thymus dna were investigated by uv spectroscopic. Original paper synthesis, characterization, dna binding properties, fluorescence studies and antioxidant activity of transition metal complexes with hesperetin2hydroxy. Synthesis, characterization, dna binding properties, fluorescence studies and antioxidant activity of transition metal complexes with hesperetin2hydroxy. Studies on dna binding to metal complexes of sal 2 trien. Review molecular and cellular mechanisms of cytotoxic. The binding of these complexes with different dna sequences has been studied using a variety of techniques, including melting points, viscometry, uvvis, luminescence, cd, itc and spr. Selective binding of transition metal complexes to dna pages.

Synthesis, characterization, biological activity and dna binding 391 where dna is the concentration per nucleotide,the apparent extinction coefficient a, was obtained by calculating a obs coii, f extinction coefficient of the complex in presence of dna and b the extinction coefficients for the fully coii complex in the fully. Binding of metallointercalators to dna is reversible and depends on the properties of the intercalating molecule. Hl c10h7o2nse, 2phenyl4selenazole carboxylic acid, phen 1,10phenanthroline 1. All the metal complexes were screened for antibacterial activities.

The photochemical dna cleavage of the two complexes is also demonstrated. Voltammetric studies of the interaction of transitionmetal complexes with dna. The results showed that the metal complexes are biologically active. In continuance of our ongoing research on dna binding and cleavage happenings of transition metal complexes, in this paper we obtainable the synthesis, characterization and dna cleavage. These complexes are lowenergy mlct absorbers and bind to dna rapidly. Bovine serum albumin interactions with metal complexes. The antibacterial activities of the complexes against five species of. In most of the cases, the metal complexes stabilised dna, bind via intercalation and their affinity with ct dna are in the micromolar range.

Design, structural characterizations, dna binding, dft calculations, molecular docking and biological studies. Studies towards elucidating the binding modes between metal. Metallointercalators with different metal centers, oxidation states, coordination geometries, and overall sizes will exhibit varying depths of insertion. A supercoiled plasmid was cleaved into an open circle by the addition. Synthesis, structural, dna binding and cleavage studies of cu. Synthesis, characterization, antimicrobial activity and. Reactivity and structural studies of metal complexes bound. The study of structural and binding features of various schiff base complexes can play an important role in better understanding of the complex in biological processes.

Mass spectrometric studies of alkali metal ion binding on. Optical photophysical techniques are widely used to study the binding model of the ligand, metal complexes, and dna but not to give sufficient clues to support a binding model. Syntheses of polypyridyl metal complexes and studies of their. Pdf metal complex interactions with dna researchgate. They are found to be potent dna intercalating and cleaving agents. Electronic absorption spectroscopy serves as the most common means to study the interactions between metal complexes and dna barton et al. As zinc being an active core of binding site of some metalloenzymes, the synthesis of structurally similar zinc ii complexes is on the offing.

Dna interaction studies of a new platinumii complex. Experimental studies suggest that the complexes bind to dna through intercalation. Dna structure can deviate from classic bform helix, and therefore be specifically recognized by a protein. Synthesis, electronic structure, dna and protein binding, dna. Synthesis, characterization, dna binding interaction. These dualfunctional metallointercalators bind to fs dna, resulting in increased solution viscosity and melting temperature. The titration absorption curve for the dna binding activity with the niiil 1 complex is shown in fig. Results indicate the dna binding affinities of complexes follow the order 3 4 1 2 5 6.

Thus through a series of experiments in this thesis, attempts have been made to correlate the structure and dynamics of metal complexes bound to dna. Basespecific hbond donor, acceptors, and nonpolar groups are recognized by dna binding proteins. Three novel transition metal complexes ml2phenh2o m mn, co, zn. Aug 26, 2009 the binding properties of the two complexes to calf thymusdna ctdna were investigated by different spectrophotometric methods and viscosity measurements together with equilibrium dialysis and circular dichroism spectroscopy. Synthesis, characterization and dna binding study of ruthentumii thiosemicarbazone complexes k. Aug 11, 2017 the interactions of the synthesized chalcone and its metal complexes with dna were studied using steady. Two adjacent guanine residues can replace the chlorines on cisplatin and generate a stable species. The experimental results show conformably that the mode of binding of the complex fesal2trienno3 to dna is nonclassical electrostatic action, but. Using supramolecular principles, we have been designing luminescent lanthanide complexes with a defined hairpin bisinterlacator in order to obtain luminescent probes ab. Transition metal complexes have attracted considerable attention as catalytic systems. Synthesis, characterisation and dna binding studies of. Prepared complexes showed enhanced dna binding tendency than the parent drug that might be of interest for future research.

Studies on dna binding to metal complexes of sal 2trien. Synthesis, characterization, dna binding properties. Aug 27, 2016 dna binding ability of these complexes was studied on ct dna by using uvvis absorption, fluorescence and viscometry. Competitive dnabinding studies between metal complexes. Synthesis, characterization, antimicrobial, dna binding and. The concentration of calfthymus ct dna was determined from the absorption intensity at 265 nm with a e value li et al. Pdf synthesis and dna binding studies of niii, coii. The effect factors on the medicinal properties of metal complexes are geometry, size and hydrophobicity of the ligands, and also the nature of metal ions. Antimicrobial, anticancer and dna binding studies of. Synthesis, characterization, electrochemical studies, dna.

Synthesis, characterization, dna binding interaction studies. Find, read and cite all the research you need on researchgate. A wellknown example of metal complexes binding to unmodified dna involves the cisplatin family of anticancer drugs. The intrinsic binding constants for npn1 and pln with dna are 2.

The data show that the complexes have composition of ml 2 type. Synthesis and dna binding study of zinc ii coordination. Metal complexes as dna intercalators accounts of chemical. All the dna binding studies reveal that they are good intercalators. Transition metal complexes have attracted curiosity due to dna binding and cleavage properties under physiological conditions. The core of bioinorganic chemistry has focused on the study of metal sites in metalloproteins and. Jayabalakrishnan2 1department of chemistry, kumaraguru college of technology, coimbatore, tn, india 2department of chemistry, sri ramakrishna mission vidyalaya college of arts and science, coimbatore,tn, india. Synthesis and characterization of metal complexes containing curcumin c 21 h 20 o 6 and study of their antimicrobial activities and dna binding properties.

Synthesis and dna binding studies of niii, coii, cuii. Structural data of dna binding and molecular docking studies. Jun 19, 2003 a series of mixed metal complexes coupling a lightabsorbing ruii terpyridine unit to a reactive cisptiicl2 moiety through polyazine bridging ligands have been prepared and their light absorbing, redox, and dna binding properties assayed. Synthesis, characterization, and dnabinding studies of. Fluorescence studies along with viscosity measurements have also been checked to authenticate the binding of metal complexes with dna.

A fisher100 infrared spectrophotometer was used to recorded the ir spectra as kbr and csi disc. Interactions of metal complexes with dna by jihan h. Synthesis, characterization and dna binding study of. These studies were further supported by molecular docking studies and the resulting binding energy of docked metal complexes 1a and 1b were found to be. Since the discovery of cisplatin, transitional metal complexes have been proven to be potent dna intercalating agents. Synthesis, characterization, dna binding properties, fluorescence studies and antioxidant activity of transition metal complexes with hesperetin2hydroxy benzoyl hydrazone. Metal complexes, cefixime, dna binding studies, ligand. The results of the cytotoxicity and dna cleavage studies are presented in this chapter. Fundamental studies of metaldna interactions and metal sensing. Dna binding studies the dna binding experiments were performed in trishcl nacl buffer 50 mm trishcl1 mm nacl buffer, ph 7. Pdf synthesis, characterization, dna binding interaction studies. The % fluorescence decrease is directly related to the extent of dna binding. From small metal complexes in biological systems to metalloproteins. Pdf synthesis and characterization of metal complexes.

Dna binding of metal complexes with mixed recognition motifs. Pdf synthesis, characterization, dna binding properties. Dna binding behaviors of these complexes with calf thymus dna ct dna have been investigated by absorption spectra, viscosity, and. Dna binding studies were also carried out for the synthesized complexes. The complexes fesal2trienno3 and cusal2trien have been synthesized and their interaction with calf thymus dna has been investigated for the first time using uv spectra, fluorescence spectra, thermal denaturation, and viscosity measurements.

The mobility of the bands decreased as the concentration of the complex was increased, indicating that there was increase in the interaction between the metal. The binding poses of the metal complexes 1 a and 2 b into the dna duplex obtained from molecular docking analysis. Post graduate and research department of chemistry, sri ramakrishna. They interact more strongly with dna and they have a higher binding constant than the other complexes, due to the possible geometry of the niii complex of the distorted. A certain degree of binding specificity was observed for metal complexes, due to weak interactions between the ligand sphere and the protein binding site, as some studies suggested that metal complexes most likely affect the trp4 residue, found on the surface of the protein, and thus more accessible. Using fret melting assays, the binding affinity and selectivity of the five metal complexes against six different g4 dna structures as well as a duplex dna have been determined. Detailed dna binding studies of these two complexes, together with three previously reported metal salphen complexes, are presented. Binding of synthesized complexes with calf thymus dna ct dna was studied by spectroscopic methods and viscosity. Therefore, viscosity measurements were carried out to further clarify the interaction of metal complexes and dna. Synthesis, crystal structure, and dnabinding studies of a. The molecular docking scores of 4, 5a, and 5b with bdna suggest a similar trend in behavior to that observed in the dnabinding study. The absorption spectra of the copper complexes were compared with and without ct dna at 400 nm fig. Studies on dna binding to metal complexes of sal 2trien zhou chengyong1,2, xi xiaoli 1, and yang pin1 1key laboratory of chemical biology and molecular engineering of the ministry of education, institute of molecular science. The tgdta study of the cobalt complex in air show that, it decomposes steadily to yield metal oxide as residue.